HP2U1042 - UGA/INSERM
The HP2 laboratory (Hypoxia and Physiopathology Cardiovascular and Respiratory Laboratory) is a research laboratory specialized in understanding the physiological, molecular and cellular mechanisms that cause cardiovascular and metabolic complications of sleep apnea syndrome (SAS). Our laboratory brings its expertise in the analysis of physiological parameters of murine models.
The fundamental research team of HP2 is localised on the health site of la Tronche, near the CHU Michallon near Grenoble. Our laboratory provides a highly multidisciplinary environment. The grouping of scientists in fondamental research, exercise, altitude physiology and clinical research for the integrated management of sleep apnea syndrome is the specificity and strength of the HP2 Laboratory.
Our team is as much interested in the physiopathological mechanisms as in the consequences of the SAS using pharmacological, biochemical and physiological investigation techniques. To reach our objectives, the team uses different approaches of physiological explorations (echocardiography, IRM, in vivo surgery techniques, study of ex vivo and in vitro models, etc.) and has developed, an intermittent hypoxia device, very efficient and unique in Europe, which exposes hypoxia to rodents and cells (the HypE platform).
Within the Arterylastic project, the HP2 laboratory brings its expertise in animal and vascular physiology.
The objectives of this working group are :
The study of the mechanism of action of SEP in vivo and ex vivo in murine models
The objective is to restore (or at least improve) the function and the mechanical properties of mouse arteries under conditions of vascular dysfunctions related to elastic fiber lesions, induced by: I) aging, II) intermittent hypoxia or III) genetic deficiency of elastin. We will evaluate the impact of SEP treatment on the main physiological parameters of mice (blood pressure …), on the arterial and cardiac structure (histology …), as well as on the reactivity and the mechanical behavior of arterial segments ( pressure arteriography …).
The study of the mechanism of action of SEP in vitro
- Thrombogenicity, inflammation and integration of SEP in the arterial elastic laminae of primocultures of vascular smooth muscle cells of different murine models
- Study of the effect of hypoxia on different cell cultures.
The animal husbandry management
The animal husbandry management that will be used throughout the consortium. Including from an administrative point of view with the drafting of the application for project authorization from the Ethics Committee in animal experimentation.
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HP2 members working on the project